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Primary and Stem Cells: Gene Transfer Technologies and Applications
Uma Lakshmipathy, Bhaskar Thyagarajan
ISBN: 978-0-470-61074-9
Hardcover
304 pages
2012
This book describes basic cell engineering methods, emphasizing stem cell applications, and use of the genetically modified stem cells in cell therapy and drug discovery. Together, the chapters introduce and offer insights on new techniques for engineering of stem cells and the delivery of transgenes into stem cells via various viral and non-viral systems. The book offers a guide to the types of manipulations currently available to create genetically engineered stem cells that suit any investigator's purpose, whether it's basic science investigation, creation of disease models and screens, or cells for therapeutic applications.
FOREWORD vii
Michele Calos
PREFACE ix
CONTRIBUTORS xi
PART I: CLONING AND GENE DELIVERY 1
1. DNA Assembly Technologies Based on Homologous Recombination 3
Billyana Tsvetanova, Lansha Peng, Xiquan Liang, Ke Li, Jian-Ping Yang, Tony Ho, Josh Shirley, Liewei Xu, Jason Potter, Wieslaw Kudlicki, Todd Peterson, and Federico Katzen
2. Multigene Assembly for Construction of Synthetic Operons: Creation and Delivery of an Optimized All-IN-One Expression Construct for Generating Mouse iPS Cells 19
Takefumi Sone, Yoko Takata, Hiroe Kishine, Taichi Andoh, Fumio Imamoto, and Jonathan D. Chesnut
3. Strategies for the Delivery of Naked DNA 37
Jennifer C. Moore and Ronald P. Hart
PART II: NONINTEGRATING TECHNOLOGIES 49
4. Episomal Vectors 51
Junying Yu
5. Nonintegrating DNA Virus 85
Chris Kemp and David Kuninger
6. Nonintegrating RNA Viruses 103
Noemi Fusaki
7. Protein Delivery 119
Xiao-Jian Han and Kazuhito Tomizawa
PART III: INTEGRATING TECHNOLOGIES 135
8. Sleeping Beauty Transposon-Mediated Stable Gene Delivery 137
Andrew Wilber, Xianzheng Zhou, Xin Huang, Dan S. Kaufman, and R. Scott McIvor
9. Integrating Viral Vectors for Gene Modifications 163
Gemma L. Mendel, Brian Paszkiet, and Rafal P. Witek
10. Bacteriophage Integrases for Site-Specific Integration 199
Bhaskar Thyagarajan
11. Improving Gene Targeting Efficiency in Human Pluripotent Stem Cells 211
Sangyoon Han, Anna McCann, Louise C. Laurent, Jeanne F. Loring, and Ying Liu
PART IV: APPLICATIONS 227
12. Modified Stem Cells as Disease Models and in Toxicology Screening 229
Catharina Ellerstr?om, Peter Sartipy, Raimund Strehl, and Johan Hyllner
13. Screening and Drug Discovery 251
Elizabeth A. Davenport and Robert S. Ames
INDEX 273
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