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推薦指數：
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- 內容介紹
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Oligonucleotide Synthesis: Methods and Applications
Herdewijn, Piet (Rega Institute, Katholieke Universiteit Leuven, Belgium)
Binding: Hardcover
Pub. Date: September 2004
Pages: 456
ISBN: 1588292339
Abstract:
A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression.
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